碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 101 / Two feeding trials were conducted to evaluate the dietary zinc (Zn) requirement for the optimal growth of hybrid tilapia with casein or soybean meal (containing 1.95% endogenous phytic acid) as a source of protein .
Experiment 1: Casein was the major protein source. Diets supplemented 12 g/kg sodium phytate (phytic acid contented 10 g/kg). Hybrid tilapia juveniles of approximate 0.93 g body weight were fed casein based diets supplemented with 0, 30, 60, 90, 120, 150, 180, 210 mg Zn/ kg diet (poviding the actuary values of 9.2, 37.3, 66.5, 99, 138, 161, 196, 226 mg Zn/ kg diet) for 8 weeks. Each diet was fed to triplicate groups of fish. Results indicated that no significant difference (p>0.05) in weight gain (WG), feed conversion ratio (FCR) and protein efficiency ratio (PER) were observed among the dietary treatments. Non-specific immunoassay in copper-zinc superoxide (Cu-Zn SOD) anion production ratio and lysozyme (Lysozyme) activity were no significant difference (p>0.05) observed among the dietary treatments. Total superoxide (total SOD) anion production ratio were lower in fish fed diets with control diet and 37.25 mg Zn/kg diet than fish fed diets with ≧ 66.52 mg Zn/kg diet. Alkaline phosphatase (ALP) activity were highest in fish fed diet with 66.25 mg Zn/kg diet (p≦0.05). Analysis of growth performance and non-specific immunoassay of the hybrid tilapia juveniles by regression moldeling that the optimum dietary znic requirement with 10 g/kg phytic acid for hybrid tilapia juveniles were 55-71 mg / kg.
Experiment 2: Soybean meal was the major protein source to evaluate the dietary zinc requirement of tilapias fed diets containing 15.5 g/kg phytic acid. Hybrid tilapia juveniles of approximate 2.89 g body weight were fed soybean meal based diets supplemented with 0, 30, 60, 90, 120, 150, 180, 210 mg Zn/ kg diet (poviding the actuary values of 31.2,70.1,101,127,166,189,227,252 mg Zn/kg diet). Each diet was fed to triplicate groups of fish. Hybrid tilapia juveniles fed diets with 127 and 166 mg Zn/kg showed higher weight gain (WG), feed conversion ratio (FCR), protein efficiency (PER) than fish fed diets with 31.2,70.1, and 101 mg Zn/kg diet (p≦0.05). Non-specific immunoassay in alkaline phosphatase (ALP) activity were no significant difference (p>0.05) observed among the dietary treatments. Copper-zinc superoxide (Cu-Zn SOD) anion production ratio and total superoxide (total SOD) anion production ratio were lower in fish diets with 31.2,70.1,101 mg Zn/kg diet than those diets with zinc (p≦0.05). Zinc concentrations in the whole-bodys increased when dietary zinc increased. Zinc concentration in the plasma were highest in fish fed diets with with 127 mg Zn/kg diet (p≦0.05). Analysis of growth performance, non-specific immunoassay, zinc concentration in the plasma and whle body zinc retention of the hybrid tilapia juveniles by regression moldeling that the optimum dietary znic requirement with 15.5 g/kg phytic acid in soybean meal for hybrid tilapia juveniles were 123-155 mg Zn/kg diet.
| Identifer | oai:union.ndltd.org:TW/101NCYU5061005 |
| Creators | Meng-Ru Li, 李孟儒 |
| Contributors | Chen-Huei Huang, 黃承輝 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 103 |
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