碩士 / 國立宜蘭大學 / 環境工程學系碩士班 / 101 / With the growing concerns of the shortage of fossil fuels, the development and application of biomass energy has attracted much attention and has become the focus of future development around the world. Butanol is an excellent organic solvent, an important chemical raw materials and new biofuels. In this study, an immobilization technique is usded to immobilize Clostridium saccharoperbutylacetonicum N1-4 in order to explore the effect of rotating speed on biobutanol production (still, 100 rpm, 200 rpm). The biobutanol production by free cells is used as acontrol. Immobilizing materials are polymers of polyvinyl alcohol and sodium alginate.
The research data show that there were no significant differences on both butanol production rate and butanol yield at different rotating speed in control group. The highest butanol concentration is approximately 3,000 mg/L. Comparing to the control group, the biobutanol production by immobilized cells is less than that by suspended cells, and the time the fermentation initiates is two days delay. The possible explanation that the immobilized cells exposed to air and soaked into boric acid during immobilization process, resulting the formation of endospores. This will reduce the biobutanol production. However, subculturing the immobilized cell can improve the biobutanol production. The result indicates the butanol concentration reachs as high as 3,500 mg/L if subcutlturing is performed. It is also found that the immobilized cells were disintegrated at 200 rpm although the maximum butanol concentration of 2,500 mg/L occurs at this rotating speed. The disintegration is mainly caused by the collapse of the sodium alginate. The experiment used citric acid as a buffer found that butanol production increased and acetone production decreased. This could be that the citric acid serves as a carbon source during fermentation, resulting the increase of butanol yield. However, citric acid concentration of up to 6,000 ppm ceased the cell growth. The value of pH4.5 inhibited the activity of subculturing immobilized cells. The optimum pH for fermentation ranged from 5.0 to 5.5. The pH of 6.0 induced the metabolic pathway towarding to the production of acetic acid and butyric acid. In this study, the highest ABE yield and the production rate for suspended cells were 0.50 g /g and 2.50 g/L/d, respectively;for immobilized cells were 0.42 g/g and 0.32 g/L/d, respectively; for subculturing immobilized were 0.48 g/g, 2.39 g/L/d, respectively.
| Identifer | oai:union.ndltd.org:TW/101NIU07515003 |
| Date | January 2013 |
| Creators | Chen,yu-chen, 陳昱辰 |
| Contributors | Chen, Wen-Hsing, 陳文興 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 165 |
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