碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 101 / Viral Nervous Necrosis(VNN) caused by a RNA virus affects the survival rate of young fries in groupers.In recent years,our lab found that the viral load is not completely proportional to the death rate of young fries.In the study,the variations between and within genotype and phenotype were investigated.
The cDNAs of two RNA genes of VNN virus from 19 young fries were sequenced and found that all these sequences belong to RGNNV class.However, genotype variations were observed in the different locations of these two RNA genes. These variations from an individual or individuals of same pond suggest that VNN viral genes are greatly diversified.
Based on the viral sequences from 10 individuals of death and live fries, the survival rate of cells infected with VNN virus may vary, and is negatively related to the TCID50 and gene replication rate. The survival rate of the phenotype expression was in agreement with cellular observation but the difference in the survival rates of young fries with same high viral load were also observed.These studies may suggest there other factors involved in the death of young fries after VNN infection.Futhermore, diversities in phenotype expression and genotype sequences were illustrated
| Identifer | oai:union.ndltd.org:TW/101NKIM0270003 |
| Date | January 2013 |
| Creators | Cheng-Hsun Li, 李承訓 |
| Contributors | Ching-Long Huang, 黃清龍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 134 |
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