碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 101 / Monascus species belong to the fungi ascomycetes and in particular to the family of Monascaceae. Monascus is a long been known for its natural pigments used to colour food, wine, and processed meats. It is also thought to have a variety of biological activities beneficial for human health. To investigate the regulatory roles of Monascus in physiological and immune responses in shrimp, we examined innate non-specific immune responses (including total hemocyte count (THC), phenoloxidase activity (PO), and respiratory bursts (RB) activity), physiological responses (including haemolymph glucose, lactate, lipids, and serum protein), and gene expressions (including lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP), prophenoloxidase (proPO), superoxide dismutase (SOD), ferritin (ferritin), and peroxinectin (PE) mRNA transcripts) of immersed Monascus at 0, 250, 500, and 1000 mg L-1 in white shrimp (Litopenaeus vannamei). The results indicated white shrimp immersed Monascus in 500 mg L-1 can maintained lower glucose, lactate, and lipid levels at 0~48 h. Immune responses significantly increased by THC, PO, and RB activity in L. vannamei. Especially immersed Monascus in 500 mg L-1 at 48 hour. Moreover, the gene expressions of LGBP, proPO, SOD, PE, and ferritin mRNA transcripts was significantly increased by Monascus. Especially immersed Monascus in 500 mg L-1 at 24 hour. This study suggests that immersed Monascus in 500 mg L-1 at 48 hour can enhance immunity and not change physiological homeostasis in white shrimp.
| Identifer | oai:union.ndltd.org:TW/101NKIMT084004 |
| Date | January 2013 |
| Creators | Ya-Ting Chen, 陳雅婷 |
| Contributors | Shu-Ling Hsieh, 謝淑玲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 109 |
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