碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 101 / The objective of this study is to establish the process conditions for preparing collagen peptides with calcium-binding (Ca-B) activity from tilapia scales. The scales of tilapia were washed, dried, and milled to obtain a powder sample. Collagen peptides with Ca-B activity were prepared from tilapia scales treated with heating, acidic treatment and commercial proteinase (Papain,Alcalase,Flavourzyme,Protase-N and Protamax). Optimal conditions in heating, acidic and enzymatic treatment were investigated and compared for their Ca-B activity. The hydrolysates from heating and acidic treatment didn’t have high Ca-B activity, and Ca-B activity decreased significantly after artificial gastrointestinal liquid treatment. Higher Ca-B activity was obtained from tilapia scales treated with papain and flavourzyme. The tilapia scales were then treated with two enzymes system, including papain and flavourzyme. The optimal conditions in two enzymes system were listed as follows: pH 7.5; 50°C; enzyme activity, 3000 U/mL for papain and flavourzyme; the amount of tilapia scales, 4%; reaction time 2 h. The secondary digestive liquid was obtained from the tilapia scales hydrolysates followed by artificial gastrointestinal liquid treatment, and Ca-B activity remained in this liquid. The secondary digestive liquid was then fractionated with hollow fiber and QHP column separation. Three fractions (named Q1, Q2, Q3) were obtained, and Q3 showed the highest Ca-B activity. Collagen peptide powder from the spray dried hydrolysates of tilapia scale exhibited similar Ca-B activity when compared with commercial casein phosphorpeptide (CPP). Two collagen peptide drinks were prepared from fish scale hydrolysates and showed good acceptability by sensory evaluation.
| Identifer | oai:union.ndltd.org:TW/101NKIMT084024 |
| Date | January 2013 |
| Creators | Chen, Zih-Yin, 陳姿吟 |
| Contributors | Kuo, Jen-Min, 郭建民 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 69 |
Page generated in 0.0018 seconds