碩士 / 國立屏東科技大學 / 生物科技系所 / 101 / Polysaccharides may contain pathogen-associated moleculer patterns (PAMPS) stimulated innate immune response in white shrimp (Litopenaeus vannamei). There are total of 5 experimental diets, each was fed to 200 shrimps per tank (initial wt: 0.84 g) for 2 months. Weight gain of shrimp fed control diet, 0.2, 0.4 and 0.6 and 0.8 g kg-1 polysaccharide containing diet increased 669.58 %, 812.58 %, 774.25%, 740.70% and 707.21% (p<0.05), respectively. Quantitative PCR analysis showed that expression mRNA level of LvToll 2, and Lvtoll 3 and interferon gamma inducible lysosomal thiol enzyme (Lv GILT) was clearly up-regulated in hemocyte, hepatopancrea, gill, muscle and stomach of both pellet feeding supplemented with polysaccharide at 20th, 40th and 60th day culturing, compared to shrimp fed control diet. In another experiment, L. vannamei, which had been fed control diet, or polysaccharide-containing diets after 60 days culturing, were challenged with V. alginolyticus at 105 colony-forming units (CFU) shrimp-1 and then placed in sea water of 25‰. The survival of shrimp fed a diet containing 0.2 and 0.4 g kg -1 after 11 days injected increased significantly, as compared to that of shrimp fed control diet. It is therefore concluded that administration of polysaccharide in the diet at 0.2 and 0.4 g kg-1 could enhance the immune ability of L. vannamei and increase its resistance to V. alginolyticus infection
| Identifer | oai:union.ndltd.org:TW/101NPUS5111033 |
| Date | January 2013 |
| Creators | Vo-Duc Duy, 武德維 |
| Contributors | Tzou-Chi Huang, 黃卓治 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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