碩士 / 國立屏東科技大學 / 獸醫學系所 / 101 / Fish columnar disease for the province of summer when high water temperature, a common fish diseases, mainly by the Flavobacterium columnare caused columnaris, often occurs in the perch, eel, tilapia and other freshwater fish farming, and caused a large number of fish deaths, some other brackish or seawater fish farmed fish also infection. Columnaris pathogen mainly affects the surface of the gill tissue and epithelial tissue, causing rot gill or rot tail. General ornamental fish owners or fish aquaculture farmers usually due to mixed infection with other diseases often to the neglect of the disease, resulting in significant losses. In order to provide effective prevention and treatment of this disease, the preliminary experiment confirmed cases for F. columnaris strains of 16SrDNA location using molecular biology diagnostic methods to detect, PCR products were purified, cloned and sequenced. DNAstar and MegAlign software reuse and foreign strains isolated from the similarity of the comparative analysis, the results of up to 99.8% of its similarity. Another of the experiment for various disinfectants currently marketed antimicrobial effectiveness testing, the diluted concentrations were 100,10 and 1ppm, using sterilization blank tablet after immersion disinfectants, flat against on the cytophaga agar at 28 ℃ on a culture after 24-48 hours, the test results show that a variety of commercially available disinfectants diluted to the concentration for the bacteria were not significantly effect. Another test of copper sulfate were diluted to 10, 5 and 0.5 ppm concentration effect after detection, the test results showed that the concentration of copper sulfate diluted by the above test for the bacteria were no obvious effect. In drug susceptibility testing, this study has been identified for several strains of F. columnaris whom antibiotic susceptibility testing, the results show Ampicillin, Amoxicillin, Cephalexin, Florfenicol, Flumequine, Doxycycline, Erythromycin, Lincomycin and other drugs have a sensitivity. However, based on existing domestic aquatic animal drug regulations, there are still many non-approved use of the antibiotic classes. Our results provide only a reference to the academic research, in clinical disease control is still required to comply with the relevant regulations.
| Identifer | oai:union.ndltd.org:TW/101NPUS5541012 |
| Date | January 2012 |
| Creators | Ming-chin Kuo, 郭明欽 |
| Contributors | Shinn-Shoung Tsai, 蔡信雄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 59 |
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