碩士 / 國立中山大學 / 海洋生物研究所 / 101 / Previous studies have shown that muscle and liver of Epinephelus malabaricus contained more EPA when the fish were fed a fishmeal-based diet with (18:3n-3/18:2n-6) ALA/LA ratio of 3 than 0.4. This result implicated that Epinephelus malabaricus might possess certain ability to synthesis EPA, and this ability is related to the relative amounts of ALA and LA in the diet. The aim of this study was understand the ability of the grouper to synthesis HUFA. Epinephelus coioides with an initial weight of 20g were fed for 10 week with six casein–based semipurified experimental diets with varying dietary ratios of α-linolenic and linoleic acid (18:3n-3/18:2n-6, ALA/LA). The ability of the grouper to synthesis EPA and DHA were evaluated. HUFA and ALA+LA accounted for 12% and 35% of total fatty acids respectively in the experimental diets containing 10% lipid. The study was a 2×3 factorial design with two dietary ALA/LA ratios: 0.4 and 3, and three HUFA treatments: none, DHA only, DHA+EPA (3:1). Fatty acid compositions in polar and nonpolar lipid of carcass, liver, muscle were significantly affect by dietary fatty acid composition. Dietary HUFA supplementation significantly enhanced (P<0.01) growth, survival and food conversion ratio of the grouper; but dietary ALA/LA ratios did not. Carcass HUFA content of the non HUFA group was significant higher (P<0.05) than that of the initial fish. Calculation of HUFA intake and deposition reveals that grouper might possess a limited ability to synthesize HUFA when their diet was depleted in HUFA but contained a certain level of PUFA. This ability however was not reflected on growth, survival and food conversion ratio in growing grouper juveniles.
| Identifer | oai:union.ndltd.org:TW/101NSYS5270012 |
| Date | January 2013 |
| Creators | Rih-Sin Jhao, 趙日新 |
| Contributors | Houng-Yung Chen, 陳宏遠 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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