碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 101 / Marine invertebrates have been found to be a rich source of bioactive secondary metabolites. Soft corals of the genus Sinularia, Lobophtum, and Clavularia were found to possess different skeletons secondary metabolites. Therefore we have investigated the secondary metabolites of the soft corals Sinularia leptoclados, Lobophtum crassum, and Clavularia viridis. Investigations on the chemical constituents of S. leptoclados collected at Dongsha Atoll of the South China Sea have led to the isolation of one known diterpenoid 1 and one new guaiane-type compound 2. Chromatographic separation of the organic extracts of the Formosan soft coral L. crassum, collected at San-Shen-Tai, led to the isolation of one known diterpenoid 3, together with three new cembranoids 4–6. In addition, the EtOAc extract of C. viridis, collected at the Green Island, yielded two new fatty acids 7 and 8, along with three known compounds 9–15.The structured elucidations of these compounds were determined on the basis of their spectroscopic analysis (IR, ESIMS, specific optical rotations, 1D NMR and 2D NMR) and by comparison of the physical and spectral data with those of the related known compounds.The cytotoxicity against of P-388 (murine lymphocytic leukemia), HT-29 (human colon adenocarcinoma), and A-549 (human lung epithelial carcinoma) cells as well as the anti-HCMV (human cytomegalovirus) activities of metabolites 2 and 4–9 were evaluated in vitro. Metabolites 4, 7 and 9 exhibited significant cytotoxic activity against A-549, HT-29 and P-388.
| Identifer | oai:union.ndltd.org:TW/101NSYS5277012 |
| Date | January 2013 |
| Creators | Yi-ying Huang, 黃怡瑛 |
| Contributors | Cheng-Yeh Duh, 杜昌益 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 258 |
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