碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 101 / The unique gene, Distal-less homeobox (DLX6) was identified in the expressed sequence taq (EST) derived from the developing tilapia brain in our lab. The cDNA full length of DLX6 was cloned. DLX6 play a role in the differentiation of nerve cell. In this study, bioinformatics were searched for EE724153.1 (DLX6), which is a gene with 2138 bp of cDNA sequence, open reading frame(ORF) is 741bp, and deduced as 246 amino acids. The protein of DLX6 was expressed in the prokaryotic system, BL21(E.coli), and purified with Ni-NTA affinity chromatography. In the present studies, DLX6, cloned from tilapia, Oreochromis mossambicus. The possibility of DLX6 can form dimer. The ORF of DLX6 was cloned, and the pEGFP-DLX6 plasmid was constructed. The distribution of DLX6 in the pEGFP-DLX6 transfected Neuro-2a cell was identified by fluorescence .
| Identifer | oai:union.ndltd.org:TW/101NSYS5277023 |
| Date | January 2013 |
| Creators | Shih-chieh Hung, 洪士傑 |
| Contributors | Ching-Lin Tsai, 蔡錦玲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 56 |
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