碩士 / 國立臺南大學 / 生物科技學系碩士班 / 101 / In this study, the Onychostoma genus, Cyprinidae, is chosen as the main research species due to the uniqueness of geographical distribution compared to other freshwater fish genus. Onychostoma is an ideal model to study the correlation between phylogeographical relationship and species distribution. In this study, four species belonging to Onychostoma are collected from mainland China, Taiwan, and Hainan Island, which are O. barbatulus, O. alticorpus, O. barbata, O. sima, and an two other species, Scaphiodonichthys acanthopterus, Microphysogobio alticorpus are used as outgroup species in this study. Ten forward and reverse primers are used in identifying each mitochondrial loci. The total complete mitochondrial DNA sequence of species were successfully obtained and submitted to NCBI GenBank. According to the comparative analysis of mitochondria genome, the total length of four Onychostoma species and two outgroup species are estimated to be 16,000 base pairs, including twelve protein-coding genes, two ribosomal RNA genes, and twenty-two transfer genes. By decoding mitochondrial genome, the derived information would be not only helpful on the establishment of molecular systematics database of ichthyology, but understand the speciation mode of Onychostoma genus as well as the relationship between phylogeography and geographic distribution.
Identifer | oai:union.ndltd.org:TW/101NTNT5111005 |
Date | January 2013 |
Creators | Chien-yu Wu, 吳健毓 |
Contributors | none, 曾登裕 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | zh-TW |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 84 |
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