碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 101 / Supplements of mannitol and vitamin C were added in diet for white shrimp (Litopenaeus vannamei). Shrimp’s survival, growth, low dissolved oxygen tolerance and antioxidant capacity. It is speculate that mannitol can substitute vitamin C in diet for white shrimp.
In order to carry out this study, three experiments were set up: experiment 1, to study the effect of vitamin C and mannitol diet supplemented on shrimp’s survival and growth parameters. Experiment 2, to evaluate the effect of vitamin C and mannitol diet supplemented on shrimp’s survival and growth parameters with a control group (without vitamin C or mannitol). Experiment 3, was assessed to investigate response of shrimp fed diet supplemented with vitamin C and mannitol and a control group on shrimp’s low oxygen tolerance and its antioxidant capacity.
Experiment 1, 2.5 g white shrimp were reared indoor 200L round plactic tank, stocking density at 0.2 /L for 8 weeks. Shrimp were fed with two different diets. Diet 1 was assessed with 200 mg kg-1 L-ascorbyl-2-monophosphate-Mg vitamin C (C), and experiment diet 2 with 200 mg kg-1 mannitol (M), with three replicate each. The result suggests no significant differences on survival, final mean weight, and weight gain, specific growth rate and feed conversion ratio in shrimp fed diet with Vitamin C and Mannitol.
Experiment 2, 9.1g white shrimp were reared indoor in 6 round plastic tank of 200L, stocking density at 0.2 /L for 10 weeks of experiment. Shrimp were fed with the same diet used in the experiment 1 and a control diet (no Vitamin C or Mannitol). The treatment did not showed significant differences on survival. The treatment C was significantly better on final weight, weight gain, specific growth rate and feed conversion ratio at the fourth week compared with other treatment.
Experiment 3, 10.2g white shrimp were reared indoor in 6 round plastic tank of 200L, stocking density at 0.2 /L for 6 weeks of experiment. Shrimp were fed with the same diet used in the previous experiments. At the end of the experiment a random sample of eight shrimp from each treatment were taken and placed in bottler fulfill with sea water. Later divided in two groups, one subjected to stress by oxygen depletion by tightly closing the bottler while the other remained as control. This study resulted that survival, time, TAS, and SOD were significantly better in shrimp fed diet with Mannitol and vitamin C compared with the control. While TAS, Mh, SOD, GLU and LAC in shrimp fed diet Mannitol were significant enhanced as compared with shrimp fed diet vitamin C. Other parameters (DO2, Mb, GPx, GR, TRI and OCR) remained comparable in all the treatments.
Based on price and the similarity performance of Mannitol and Vitamin C, it’s concluded that Mannitol at 200mg can replace vitamin C at the same portion in diet for 2 ~ 12g white shrimp.
| Identifer | oai:union.ndltd.org:TW/101NTOU5086018 |
| Date | January 2013 |
| Creators | Chun-Chieh Chiu, 邱俊傑 |
| Contributors | Yew-Hu Chien, 陳瑤湖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 71 |
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