碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 101 / Abstract
This study investigated the effects of dietary ractopamine hydrochloride on the growth, body composition and muscle residues of white shrimp, Litopenaeus vannamei. There were three experiments : (1)To investigate the effects of 0, 2, 4, 6 and 8 mg/kg of ractopamine on growth of white shrimp. (2)To investigate the effects of diets included 25, 35 and 45% protein and supplemented of ractopamine 0 and 6 mg/kg on growth of white shrimp. (3) To investigate the effects of diffent size shrimp fed with diets containing 6 mg/kg ractopamine on the shrimp hepatopancreas residue.
Diets were formulated of 38.2% crude protein, 14% crude lipid, were supplemented with 0, 2, 4, 6 and 8 mg/kg of ractopamine in the experiment I. The duration of the study was 6 weeks. White shrimp fed with diets included 6 and 8 mg/kg ractopamine had significantly higher weight gain than those fed the control diet. White shrimp fed with diets included 6 and 8 mg/kg ractopamine showed no significantly different weight gain. White shrimp fed with diet included 8 mg/kg ractopamine had significantly higher muscle protein than those fed other treatment diets. The lipid level of muscle of white shrimp decreased with increasing dietary ractopamine level. There is no ractopamine residues found in the muscle of white shrimp. White shrimp fed with diets included 0, 2, 4, 6 and 8 mg/kg ractopamine after 6 weeks contained 0, 0.65, 1.49, 1.91 and 2.58 μg/g ractopamine in the hepatopancreas.
Diets in experiment II were designed to contain 25, 35 and 45% protein level supplemented with 0 and 6 mg/kg ractopamine, respectively. The duration of the study was 6 week. White shrimp fed with diets included 25、35 and 45% protein and supplemented 6 mg/kg ractopamine had significantly higher weight gain than those fed different protein level without ractopamine. White shrimp fed with diets supplemented ractopamine had significantly higher protein of muscle than those fed diets without ractopamine. The lipid level of muscle of shrimp fed diets containing ractopamine is lower than that of shrimp fed without ractopamine. There is no ractopamine residues found in the muscle of white shrimp. White shrimp fed with diet included 25, 35 and 45% protein, and supplemented 6 mg/kg ractopamine fed detected 1.17, 1.49 and 1.21 μg/g ractopamine in the ahrimp hepatopancreas.
In experiment III, the effects of 6 mg/kg dietary ractopamine on the ractopamine accumulation and discharge rate of different size of shrimp. HPLC was used to detect concentration of ractopamine in shrimp hepatopancreas after fed diet with ractopamine at day 0, 1, 3, 7 and 14, and the concentration of ractopamine in shrimp hepatopancreas after stop feeding diet with ractopamine at day 15, 17, 19, 21, and 28.The amount of ractopamine of 4g and 1g shrimp fed diet with 6 mg/kg ractopamine could not be detected in hepatopancreas on day 0. On day 1, 3, 7 and 14, ractopamine detected in 4g shrimp hepatopancreas were 1.27, 1.65, 2.81 and 3.26 μg/g, and 1g shrimp hepatopancreas were 0.78, 1.41, 1.80 and 2.04 μg/g respectively. After stop feeding of diet containing ractopamine for 15, 17, 19, 21 and 28 days, concentration of ractopamine detected in hepatopancreas of 4g shrimp were 2.35, 2.29, 1.95, 0.79 and 0 μg/g. However, concentration of ractopamine in the hepatopancreas of 1g shrimp were 1.50, 1.04, 0.62, 0 and 0 μg/g.
| Identifer | oai:union.ndltd.org:TW/101NTOU5086026 |
| Date | January 2013 |
| Creators | Shan Lin, 林山 |
| Contributors | Shyn-Shin Sheen, 沈士新 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 48 |
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