碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 101 / During late blastula stage, dorsal BMP signaling induces ΔNp63 expression to develop epidermal ectoderm. The development of keratinocytes and non-keratinocytes including
different ionocytes such as Na+, K+-ATPase- rich cells (NaRCs) and H+-ATPase-rich cells (HRCs) as well as mucous cells is regulated by Delta-Notch lateral inhibition
signaling. Previous studies demonstrated that Foxi3a and Foxi3b play essential roles in the specification of ionocytes, while the regulation of mucous cell development is still unclear. Previously, we found that Sox10 regulated agr2 expression in the otic vesicles by morpholino antisense oligomer knockdown. Surprisingly, we indentified decreased
numbers of agr2- or pvalb8-positive mucous cells in sox10 morphants during 24 to 48 hpf. However, knockdown of sox10 does not affect grhl1 expression. In addition,reduced numbers of ecac-positive NaRCs or atp6v1al- positive HRCs were detected in 24 hpf sox10 morphants. Furthermore, we discovered expressions of foxi3a or foxi3b that are important transcription factors regulating ionocyte development were affected by knockdown of sox10. Reduced numbers of foxi3a-positive or foxi3b-positive inocytes were identified in sox10 morphants at 5 somites and 24 hpf. In order to investigate the epistatic relationship between sox10 and foxi3a/foxi3b, we conducted rescue experiments. Recovery of numbers of ecac-positive NaRCs or atp6v1al-positive HRCs were detected in embryos that had been co-injected with sox10 MO and either foxi3a or foxi3b mRNA at 24 hpf. These results indicate that Sox10 may regulate
expressions of foxi3a or foxi3b to modulate the development of NaRCs or HRCs. However, the development of keratinocytes was not affected by knockdown of sox10. In addition, prevention of BMP signaling by treatment with different antagonist showed that BMP signaling regulates the development of ionocytes and mucous cells but Sox10
is not involved. Previous studies demonstrated that Sox10 regulates the differentiation of neural crest cells to develop into cartilage, neuron and pigment. While this study
provides a novel role of Sox10 that Sox10 not only regulates the differentiation of neural crest cells but also modulates the development of epidermal ionocytes and mucous cells in zebrafish embryos.
| Identifer | oai:union.ndltd.org:TW/101NTOU5613007 |
| Date | January 2013 |
| Creators | Chen-Hsiu Li, 李健脩 |
| Contributors | Sheng-Ping L.Hwang, 黃聲蘋 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 76 |
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