碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 101 / The interaction between basic helix-loop-helix (b-HLH) factors and home-domain (HD) factors is required for retinal development. NeuroD is one of the b-HLH factors, which regulates processes of cell cycle withdraw during retinal differentiation. The hypoxia induce factor (HIF) is a member of the basic helix-loop-helix (b-HLH) / Per-ARNT SIM domain family, and its structure is stabilized under hypoxia stress. In vertebrate embryos, HIF factors, including HIF1α and HIF2α, play critical functions to ensure tissue differentiation. For instance, HIF1α mediates cardiovascular development in zebrafish and HIF2α affect pancreas development in mice. Birc5a/-5b (Survivin) is anti-apoptosis protein, that has high level of expression in various tumor cells and it has been used as a cancer marker gene. In zebrafish embryios, Hif2α controls CNS development through the regulation of birc5a/-5b expression. Previously, it was shown that knockdown hif2α or birc5a expression abrogated retinal differentiation. In addition, knockdown hif2α reduced birc5a/-5b and neuroD transcriptions. In addition, knockdown birc5a/-5b also inhibited neuroD transcription. It raises a possible correlations between hif2α, birc5a/-5b and neuroD to mediate retina development . In this study, I demonstrate that knockdown hif2α inhibits both birc5a/5b and neuroD transcription and abrogates CNS and retinal cell differentiation. In hif2α morphant, ectopic birc5a/-5b cRNA could rescue neuroD transcription effectively and ectopic neuroD cRNA could rescue birc5a/-5b transcription successfully. Consequently, the CNS and retinal cell differentiations are all rescued significantly. In conclusion, we have demonstrated that in zebrafish embryos, the CNS and retinal neuronal differentiation is mediated by a strong coordinated regulation of neuroD and surviving under the control hif2α.
| Identifer | oai:union.ndltd.org:TW/101NTOU5613020 |
| Date | January 2013 |
| Creators | Yu-Ling Lin, 林鈺玲 |
| Contributors | Chin-Hwa Hu, 胡清華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 43 |
Page generated in 0.0064 seconds