碩士 / 國立臺灣大學 / 食品科技研究所 / 101 / Neuroinflammation may play a role in the pathogenesis of Parkinson’s disease (PD), which is a common neurodegenerative movement disorder caused by the loss of nigral dopaminergic neurons. Major current treatment of PD is L-dopa, which can be the substitute of dopamine but unable to supress the neurodegeneration in the brain. Therefore, searching for components which can prevent the loss of dopaminergic neurons via regulating neuroninflammation may be a new strategy for the treatment or prevention of PD in the future. Chlorella pyrenoidosa is a single-celled alga often used as dietary supplement, with high nutritional value such as protein, vitamin, linolenic acid and dietary fiber. Polysaccharides from C. pyrenoidosa possessed great immunological activities, including anti-inflammatory and immune enhancement effects. However, its neuroprotective effects was not fully understood. Based on the proof that peripheral and brain inflammation are revelant, the objective of our study was to investigate the effects of C. pyrenoidosa polysaccharides (CPS) in a mouse model of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced PD. In addition, our study screened for the polysaccharides fraction with the greatest immunomodulatory activities through LPS-induced inflammation model in RAW 264.7 cell. Our results indicated that CPS showed neuroprotective effects by reducing the behavioral deficits, increasing the contents of striatal dopamine (DA) as well as its metabolites DOPAC and HVA, and inhibiting DA turnover ratio, tyrosine hydroxylase loss as well as microglial activation. Moreover, CPS suppressed the production of serum cytokines TNF-α, IL-1β and IL-6 and enhance the peripheral immunomodulatory biomarkers such as serum diamine oxidase and small intestinal secretory immunoglobulin A in PD mice. In cell model, our research showed that Chlorella indigestive polysaccharides (CIP) and its chromatography fractions decreased LPS-induced nitric oxide (NO) and PGE2. Furthermore, the large MW acidic polysaccharides (LMA) from CIP had the greatest anti-inflammatory effects, including the inhibition of NO, PGE2 and cytokines TNF-α, IL-1β and IL-6 levels in LPS-induced RAW 264.7 cell. Taken together, these findings demonstrated that CPS could delay the disease progression in PD via its immunomodulatory action, and LMA might be its active component.
| Identifer | oai:union.ndltd.org:TW/101NTU05252033 |
| Date | January 2013 |
| Creators | Phoebe Beverly Chen, 陳品如 |
| Contributors | Lee-Yan Sheen, 沈立言 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 184 |
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