碩士 / 國立臺灣大學 / 海洋研究所 / 101 / Polyhydroxyalkanoates (PHAs) are polyesters which can be produced by various microorganisms. Because of the properties of biodegradability, biocompatibility and thermoplasticity, PAHs have become a bio-plastics material applied in many industrial fields in recent years. They have also gained a great attention of scientists in basic researches and industrial applications. Polyhydroxybutyrate (PHB), a PHA, is a polymer of 3-hydroxybutyrate (3-HB). It was found only synthesized by prokaryotes. 3-HB could be used for growth of many bacteria of different taxa as the sole carbon source. The aims of this study were to screen and explore the bacteria which could utilize 3-HB as a sole carbon source and those which could further accumulate PHB. The samples were obtained from Taiwan north coastal seawater and cultured in glucose mineral medium (GM medium) and 3-HB mineral medium (HBM medium) after serial dilution. The abundance of bacteria, which could utilize glucose or 3-HB as a sole carbon source, was calculated by most-probable-number method (MPN). The amounts of culturable bacteria were found ranged from 2.4×102 to 1.3×104 cells/ml in GM and 3.3×102 to 1.1×104 cells/ml in HBM. Forty-five isolates were found able to utilize 3-HB. They were motile and Gram-negative. All isolates required NaCl for growth and grew at 4-45 °C. According to 16S rRNA gene sequence analysis, most isolates belonged to Pseudoalteromonas and Alteromonas, and others were Marinobacterium, Vibrio and Ruegeria. Isolates A3 and A23, belonging to Vibrio genus, were found able to accumulate PHB as revealed by Sudan Black B staining.
| Identifer | oai:union.ndltd.org:TW/101NTU05279020 |
| Date | January 2013 |
| Creators | Pei Ling Lin, 林姵伶 |
| Contributors | 謝文陽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 88 |
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