碩士 / 國立臺灣大學 / 動物學研究所 / 101 / In the thesis, we explored the role of white spot syndrome virus (WSSV) immediate early gene (IE1) in prophenoloxidase activating system. The SDS-PAGE results revealed that 10% glycerol Buffer A could stabilize WSSV IE1 purification. After far-western blotting analysis, it revealed that WSSV IE1 could interact with transglutaminase, prophenoloxidase and hemocyanin. We validate temporal phenoloxidase activity in Penaeus vannamei after WSSV viral particle injection, it indicated that phenoloxidase activity in Penaeus vannamei could be upregulated in 4 hours and downregulated in 8-12 hpi. According to the phenoloxidase activity assay, it revealed that WSSV IE1 could suppress phenoloxidase activity in Penaeus vannamei; WSSV364 was used as negative control in phenoloxidase activity assay. According to the phenoloxidase activity in Penaeus vannamei with WSSV IE1 pretreatment, it could be concluded that IE1 inhibited prophenoloxidase activating pathway. It suggested that IE1 could have suppressive function in prophenoloxidase activating pathway.
| Identifer | oai:union.ndltd.org:TW/101NTU05312020 |
| Date | January 2013 |
| Creators | Fang-Yu Yuan, 袁芳煜 |
| Contributors | 羅竹芳 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 56 |
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