碩士 / 國立臺灣大學 / 植物科學研究所 / 101 / Oncidium Gower Ramsey is one of the most important horticulture export product in Taiwan. Most of species from Orchidaceae family, including Oncidium Gower Ramsey, are originated in tropical areas Thus they have several special structures corresponding to drought environments.
Plant constantly challenged by external abiotic stress including drought, cold and salinity. In order to adapt to osmotic unbalance resulted from these abiotic stresses, plant evolved several special mechanisms to adjust its internal ion distribution such as NHXs cation/H+ antiporters. In previous studies, NHXs had considered as important regulators in respond to salinity stress.
We identified two putative NHX genes from Oncidium Gower Ramsey named “OgNHX1” and “OgNHX6”. These two genes expressed equally in different parts of Oncidium tissues, and surprisedly, unchanged under external salt or hydrogen peroxide treatment. The phenotypes of transgenic Arabidopsis overexpressing OgNHX1/6 showed no differentces comparing to wild-type plants when grown in salnity environment. However the results from stage-dependent realtime-PCR and promoter-GUS analysis suggest possible connections between OgNHXs and plant development. Our results suggest the functions of OgNHX1 and OgNHX6 are atypical to pervious studies, in which consider NHXs as efficient candicates to generate salt-tolerant plants.
| Identifer | oai:union.ndltd.org:TW/101NTU05366014 |
| Date | January 2013 |
| Creators | Shun-Te Wang, 王順德 |
| Contributors | Kai-Wun Yeh, 葉開溫 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 94 |
Page generated in 0.023 seconds