碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / The freshwater cyanobacterium Microcystis aeruginosa has been extensively studied. The use of strain M‧TY-1in our laboratory is to produce MC-LR. However, the fractions without MC-LR was not studied. It is known that Microcystis aeruginosa produces many classes of natural products. Several hypotheses on the function of these compounds in the physiology and ecology of cyanobacteria have been discussed, mostly related either to grazing protection or to allelopathy. Here we investigated the chemical
components of M‧TY-1 for their structures and possible activities.
Two micropeptins and three monogalactosyl monoacylglycerols (MGMGs) was isolated and structure determinated on the basis of spectroscopic analysis. They are Micropeptin TY1046 (1), Micropeptin TY1042 (2), 1-O-(4,7,10,13-hexadecatetraenoyl)-3-O-β-D-galactopyranosylglycerol (3), 1-O-(6,9,12-octadecatrienoyl)-3-O-β-D-galactopyranosylglycerol (4) and 1-O-(7,10-hexadecadienoyl)-3-O-β-D-galactopyranosylglycerol (5). Compound (1) and (2) may exhibit the inhibitory of chymotrypsin. In addition, the proton NMR data of compound (1) in DMSO indicated an intermolecular hydrogen bond within the ring structure of micropeptins. compound (3), (4) and (5) are iNOS inhibitors. Their IC50 are 42.16 μM, 39.24 μM, and 27.85 μM， Decreasing unsaturation of the fatty acid side chain enhances the NO inhibitory activity.
| Identifer | oai:union.ndltd.org:TW/101NTU05451015 |
| Date | January 2013 |
| Creators | Tian-Jiun Yu, 余天峻 |
| Contributors | 周宏農 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 63 |
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