碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / Fish face harsh and fluctuating ion composition in fresh water, therefore it is
important that fish have to develop the regulatory mechanisms for ion homeostasis.
Vitamin D3 is a group of fat-soluble secosteroids. It functions as a hormone and
participates in many physiological reactions in animals.
Vitamin D3 has been documented to be an endocrine of regulating ion uptake in
mammals, however, the role of vitamin D3 on the different ions is still unclear in
teleosts. In the present study, we focus on whether vitamin D3 controls the regulation
of different ions. Since ionocytes primarily regulates ion transport in embryonic skin
and/or adult gills of fish, we further explore whether vitamin D3 affects the
proliferation or differentiation of ionocytes in teleosts. Plenty of genetic databases and
mutants as well as the applicability of many molecular/cellular physiological
approaches are advantages in using zebrafish (Danio rerio); a good model to
investigate the molecular physiological mechanisms of epithelial ion uptake by
vitamin D3 in teleosts.
First, we observed whole-body content of Ca、Na and Cl as well as the expressions
of ionocyte-related genes were up-regulated after vitamin D3 treatment. Subsequently,
we further study the regulatory mechanism for ionocytes by vitamin D3. The data
showed that vitamin D3 stimulated the differentiation of ionocytes by increasing the
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expression of foxi3a, and the ionic regulation by vitamin D3 were mediated by vitamin
D3-VDR signaling in zebrafish.
Taken together, we demonstrated that vitamin D3controls the regulation of
ionocytes differentiation through modulating activities of foxi3a to increase ionocyte
densities, as well as the expressions of ion transporters and ultimately regulate the
functions of ionocytes.
| Identifer | oai:union.ndltd.org:TW/101NTU05451021 |
| Date | January 2013 |
| Creators | Huei-Jyun Hu, 胡惠鈞 |
| Contributors | 黃鵬鵬 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 49 |
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