碩士 / 國立臺灣大學 / 醫學工程學研究所 / 101 / Post-surgical adhesion remains such a severe problem that patients suffer complications such as bowl obstruction, chronic abdominal pain and infertility. However, the usage of the relative commercial products is limited such as the bloodless condition during surgeon. The price is high due to the value of the materials. For reducing the expenses of the anti-adhesion products, we are trying to use chitosan film, fucoidan/chitosan film and graphene oxide/chitosan film in anti-adhesion application. The standing point we are concerned about is cell anti-adhesion properties of these materials. Chitosan accelerates wound healing process and possesses anti-bacterial property. Fucoidan added in the hyaluronic acid film lower the adhesion grade with anti-inflammation, anti-angiogenesis properties. Graphene oxide owns anti-bacterial property and biocompatibility with limited content. We combine fucoidan with little chitosan content to catch more water-soluble fucoidan and let the mixture coat on chitosan film. On the other hand, graphene oxide we prepared is qualitatively proved by Fourier transform infrared spectroscopy (FTIR) to get carbonyl, carboxyl and hydroxyl group. The size of graphene oxide is 10μm observed by transmission electron microscopy (TEM). The addition of the fucoidan and graphene oxide is used to decrease the affinity of NIH-3T3 fibroblasts based on the sulfur functional group and chiefly carboxyl group respectively. Following from that, the surface roughness evaluated by atomic force microscopy (AFM) with addition of second material is approximately 320nm, which is twice as rough as the pure chitosan film. The contact angle test with 30-40 degree reduction also tells the additional materials raise the hydrophilicity of film surfaces, which assists the film adhesion to the wound area during the abdominal surgeries. Cell viability by MTT assay test are all getting up to 90%, which would not be an issue in each kind of film, even with limited graphene oxide content. NIH-3T3 fibroblasts tend to inhibit the linkage from chitosan film with fucoidan or graphene oxide rather than pure chitosan film as a result of the interaction of surface roughness and functional groups through MTS assay tests and cell image by optical microscopy (OM), fluorescence microscopy (FM) and scanning electron microscopy (SEM). Animal study evaluation shows that chitosan film lower the adhesion grade from 3 to 2.3. The fucoidan/chitosan film and graphene oxide/chitosan film are obviously decreased the tissue adhesion score to 0-0.3. Additionally, residues of films cannot be found near the cecum wound, which means the biodegradability of the film within the wound healing process. H&E stain reveals the adhesion layer disappears when adding fucoidan or graphene oxide in chitosan film. These results firmly suggested that the fucoidan/chitosan film and graphene oxide/chitosan film are favorable biomaterials in application of post-surgical adhesion.
| Identifer | oai:union.ndltd.org:TW/101NTU05530017 |
| Date | January 2013 |
| Creators | Guan-Wei Chen, 陳冠維 |
| Contributors | 黃義侑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
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