碩士 / 國立臺灣大學 / 分子暨比較病理生物學研究所 / 101 / Aabalone herpesvirus （AbHV） infection of cultured abalone Haliotis diversicolor supertexta has induced high mortality in Taiwan in 2003. The histopathologic study has revealed AbHV is neurotropic and has caused ganglioneuritis in moribund abalone. In this study, an in situ hybridization protocol was developed to detect AbHV gene distribution within the host to verify its pathogenesis. This study confirmed that the expression pattern of AbHV was restricted to neurons as described previously by light and transmission electron microscopy, and confirmed this is a neurotropic virus.
Another abalone virus disease is suspected in Taiwan recently. This undetermined virus （AbV2） can be amplified gene fragment in specific length by primer designed from the conserved region of herpesvirus and further sequencing revealed this virus is belonged to members of the Herpesvirales, however, the identity was low. ISH protocol is followed by aforementioned one. The result revealed AbV2 was restricted to hemocytes and suggested this is a hemocytropic virus. Retrospective study revealed AbV2 may have already existed in the farms before the first outbreak of AbHV.
| Identifer | oai:union.ndltd.org:TW/101NTU05628002 |
| Date | January 2013 |
| Creators | I-Wen Chen, 陳怡彣 |
| Contributors | 張本恆 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 48 |
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