碩士 / 國立臺灣科技大學 / 化學工程系 / 101 / Cyanophycin granule polypeptide (CGP) is a non-ribosomal synthesis protein. The polymer consists of an aspartic acid backbone with arginine and lysine as side chains. Gelatin is composed of partial hydrolysis of collagen, and is the product of almost continuous repeats of the glycine- proline -hydroxyproline sequence.
Gelatin and CGP have good biocompatibility. However, the strength and toughness of CGP is poor, which limits its applications. The gelatin has good hydrophilicity, film forming and mechanical properties. In this study, we prepared different ratios of gelatin/CGP composite scaffolds by cross-linking reaction with glutaraldehyde in an effort to improve the mechanical properties of the composite material and the growth of skin fibroblast.
We mixed different ratios of gelatin and CGP in solution and the mixture was cross-linked with glutaraldehyde. We used scanning electron microscopy (SEM) to observe the surface, cross-section and cell attachment morphology of the composite scaffolds. A universal test machine was used to detect the tensile strength and elongation of the material. The decomposition temperatures were measured by thermogravimetric analysis(TGA), and the hydrophilicity was examined by a contact angle meter. The growth of skin fibroblast was monitored by MTT assay.
The results showed that the pore and layer structures became more evident with an increasing percentage of CGP. The mechanical test showed that the mixture of CGP and gelatin can improve the tensile strength at an optimal ratio of gelatin and CGP . The composite of soluble CGP cross-linked with gelatin showed two decomposition temperatures by TGA analysis. The composites also provided a better cell growth environment for skin fibroblast on the gelatin/CGP composite scaffolds.
In conclusion, the composite of CGP cross-linked gelatin can improve the tensile strength and the growth of fibroblasts which extend the applications of CGP.
| Identifer | oai:union.ndltd.org:TW/101NTUS5342101 |
| Date | January 2013 |
| Creators | Jhe-yu Li, 李哲宇 |
| Contributors | Wen-chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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