The Recognition of Chlamydomonas Reinhardtii on Algae-Imprinted Polymer Coated Electrodes to Enhance Its Hydrogenase Expression for Microbial Fuel Cells / 製備具有萊茵衣藻辨識性的藻類拓印高分子電極以增加氫化酶之表現並應用於微生物燃料電池

碩士 / 國立高雄大學 / 化學工程及材料工程學系碩士班 / 101 / Hydrogen can be produced using microorganisms (e.g., bacteria and algae). The direct conversion of hydrogen and the transferred electrode of metabolism to electricity via microbial fuel cells(MFCs) may be a lot of benefit such as carbon dioxide fixation, low cost and little affected by environment. However, the key point of the technology is the immobilization and catalysis between microbes and electrodes. In this work, the different weight % of 38mol% poly(ethylene-co-vinyl alcohol)(EVAL), was coated on the electrode, and green algae was microcontact imprinted onto the EVAL film(algae imprinted polymer, AIP). The character of film have been analyzed by Scanning electron microscopy(SEM) and Energy-dispersive X-ray spectroscopy (EDX). The application of AIP electrodes in MFC is the second study. Their electrochemical character has analyzed by Potentiostat. The complex in algae body and hydrogenase gene expression has also been studied. Finally, there are high density and clear image of cavities on 5, 7 and 10 wt% AIP electrodes. 7 wt% AIP electrodes was the best effect in MFC. It enhanced the maximum power from 0.7 to 1.8 mW/m2. It’s around 2.6 fold. Furthermore, the expression of hydrogenase gene increased 1.15 fold after algae immobilized on AIP electrode. It shows AIP electrode can induce hydrogen production of algae and that may be one of the reasons of efficiency increasing in MFC.

Identiferoai:union.ndltd.org:TW/101NUK05063005
Date January 2013
CreatorsMing-yuan Lai, 賴名園
ContributorsHung-yin Lin, Mei-hwa Lee, 林宏殷, 李玫樺
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format110

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