碩士 / 靜宜大學 / 食品營養學系 / 101 / Presbycusis might associate with the age-related cell death and nerve function degeneration in auditory system. Spirulina platensis was demonstrated to have many health beneficial effects, such as anti-aging and neuroprotection. However, the effect of Spirulina platensis on presbycusis is still unclear. This study was aimed to examine the effects of Spirulina platensis on the presbycusis and the related mechanism, such as regulatory factor of cell death and neurotrophic factors. 11-month-old senescence accelerated mice (SAM) was used in this study and was divided into SAMR1 control, SAMP8 control and Spirulina platensis (400 mg/Kg BW) SAMP8 groups. The auditory brainstem response (ABR) test was performed at the pre- and post- six feeding weeks. After sacrificed, we evaluated the cochlear histology, the density of spiral ganglion neurons and numbers of hair cell in organ of Corti. The poly ADP-ribose polymerase-1(PARP-1) and apoptosis inducing factor (AIF) protein expressions of brain stem, temporal lobe and cochlear were analyzed. Moreover, we also examined the autophagy marker ─ microtubule associated protein light chain 3 (LC3) and neurotropic factor ─ brain-derived neurotropic factor (BDNF), neurotrophin-3 (NT-3), tyrosine kinase receptor (Trk B and Trk C) mRNA expressions of brain stem, temporal lobe and inner ear. The results showed that the ABR threshold shifts were significantly increased in the SAMP8 control group when compared with the SAMR1 control group. The supplemention of Spirulina platensis could improve the ABR threshold, prevent hearing degeneration, and significantly decrease the hair cell and spiral ganglion degeneration in middle and basal turn of the cochlear. Spirulina platensis adminstratoin also could lower the PARP-1 and AIF expressions in brain stem, hair cell and spiral ganglion neurons of cochlear, whereas the AIF expression did not differ in temporal lobe. In addition, Spirulina platensis significantly decreased the LC3 mRNA expression, and increased the BDNF, NT-3, TrkB and Trk C mRNA receptor expressions in brain stem, temporal lobe and inner ear. In summary, the supplemention of Spirulina platensis might lessen the aging related cell death by reducing the apoptotic protein and autophagic mRNA expressions of auditory system, and also could enhance neuroprotective effects by up-regulating the neurotrophic factor and it’s related receptor, therefore retarded the process of presbycusis.
| Identifer | oai:union.ndltd.org:TW/101PU000255008 |
| Date | January 2013 |
| Creators | Lin, Meihsiao, 林美孝 |
| Contributors | Chan, Yinching, Hwang, Juenhaur, 詹吟菁, 黃俊豪 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 116 |
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