碩士 / 靜宜大學 / 食品營養學系 / 101 / Nephropathy is a common microvascular complication in diabetic patients. Many antioxidative nutrients and fish oil have been reported to ameliorate the progression of various renal diseases. In this study, we examined the anti-oxidative and anti-inflammatory effects of a mixed antioxidant with fish oil (MAFO) in kidneys of STZ-induced diabetic rats. Sixty Wistar rats were randomly assigned into four experimental groups, including normal control, diabetic control, diabetic rats with low dose (75g MAFO/kg feed) of MAFO, diabetic rats with high dose (150g MAFO/kg feed) of MAFO. After 12 weeks of feeding period, concentration of fasting glucose, adiponectin, renal function (BUN、creatinine、Cys C、Ccr、L-FABP、NAGase and UAE), pro-inflammatory markers (NF-κB p65、IL-6、TNF-α、MCP-1 and CRP) and anti-oxidative markers (ROS、HO-1、GSH and AGEs) were measured. Histological study of kidneys and coronary arteriole also was performed. The results of this study showed that MAFO supplementation significantly decreased serum creatinine, CRP and renal AGEs concentration, increased renal GSH content in diabetic groups (p＜0.05). MAFO at 15% further increased renal HO-1, and diminished ROS production (p＜0.05). In addition, MAFO at 15% intake also significantly decreased TNF-α and MCP-1 in diabetic rats (p＜0.05). Renal histological studies showed that MAFO supplementation decreased AGEs accumulation and proteinuria in glomerulus of diabetic rats. In conclusion, these results suggest that high dose of MAFO could attenuate diabetic associated nephrotic oxidative and inflammatory stress.
| Identifer | oai:union.ndltd.org:TW/101PU000255019 |
| Date | January 2013 |
| Creators | Chen, Jochen, 陳若蓁 |
| Contributors | Chan, Kungchi, 詹恭巨 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 104 |
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