碩士 / 實踐大學 / 食品營養與保健生技學系碩士班 / 101 / To understand the health function of astaxanthin from Pomacea canaliculata extract, the extract was combined with protein which named astaxanthin protein powder (APP). Then fed chickens of astaxanthin can obtain astaxanthin egg yolk powder (AEYP). In this study, analysis content composition powder and function for AP and AE according to AOAC methods. The composition content, cholesterol and astaxanthin concentration, are analysis about astaxantin protein powder (APP). The cholesterol and astaxnathin concentration, fatty acid composition are analysis about astaxnathin egg yolk powder (AEYP). In animal study, the sixty-four hamster are purchased from National Animal Experimental Center, then divided into eight groups, B (Basic Diet), BC (Basic Diet with 0.2 % cholesterol), low, medium, high dose astaxanthin protein powder (4, 8, 20 mg APP/Kg BW/day) and low, medium, high dose astaxanthin egg yolk powder (1, 1.5, 2 AEYP/Hamster/day). All animals are sacrifice after six weeks and analyse lipid profile and antioxidant enzyme activity. The results show that APP contain 17.00±0.12% water, 39.00±0.98% crude protein, 12.00±1.21% crude fat, 0.86±0.01% crude fiber, 10.00±0.09% ash, soluble non-nitrogen compound 21.14±0.71%, cholesterol 0.86% and astaxanthin 1.00%. The composition of AEYP, astaxanthin is 1.25 mg% of egg yolk weight. Liver triglyceride to each group are 36.42±3.57 mg/g liver, 37.26±5.73 mg/g liver and 33.75±2.88 mg/g liver of 4APP, 8APP and 20 APP, were significantly lower than that of BC group 47.49±5.25 mg/g liver. Plasma MDA concentration of 4APP and 8APP groups that was significantly lower than BC group about 27% and 24.9%, respectively. In liver, it shows liver vitamin C concentration of 8APP group that were significantly higher than BC group abuot 12.9%. Liver catalase activity of 1.5AEYP and 2AEYP groups that were significantly higher than BC group 34% and 46.4%, respectively. Liver vitamin E contration in 20 APP group is significantly higher than BC group. Liver reducing power of 4APP、8APP、20APP that were significantly higher than BC group about 40.5%、39.11%、44.05%, respectively. From the results, astaxnathin protein powder (APP) astaxanthin egg yolk powder (AEYP) can improve concentration of plasma lipid peroxide marker malondialdehyde (MDA) and enhance liver antioxidant enzyme activity.
keywords：Pomacea canaliculata, astaxanthin, malondialdehyde, catalase, reducing power
| Identifer | oai:union.ndltd.org:TW/101SCC00255013 |
| Date | January 2013 |
| Creators | Lin, Ya-Ting, 林亞葶 |
| Contributors | Liu, Li-Yun, 劉麗雲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 110 |
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