碩士 / 大仁科技大學 / 生物科技研究所 / 101 / Coral reef ecosystem is a nutrient poor marine ecosystem which anemone and zooxanthellae symbiosis is an important biological phenomena. However, mechanisms of symbiosis of anemones and zooxanthellae and their interaction are not yet fully understood. The aim of this research was to explore the anemones response, which when symbiotic algae unable to carry out photosynthesis and provided nutrients to anemones. Three approaches for comparison in the state: (1) light and feeding the Artemia sp.; (2) complete darkness and feeding the Artemia sp.; and (3) complete darkness and not fed. The results showed the anemone’s weight, protein count, alga density, photosynthetic capacity (Fv / Fm) began to decline from the first 6 days to 24 days in darkness and feeding, its weight, protein count, alga density, photosynthetic capacity (Fv / Fm) decreased by 5%, 8%, 60%, 20% ( p < 0.05 ), but the same in the darkness and feeding of host which protein reduction of only 30% ( p < 0.05 ), despite a decrease in the amount, but did not like the no more than feeding anemone to many, and there alga density and photosynthesis capacity (Fv / Fm) compared to the control, there was no significant difference ( p > 0.05 ). Futher comparison anemones biochemical through 24 days indicators showed that glutamate dehydrogenase, total free amino acids and essential amino acids are significantly different ( p < 0.05 ), in the dark with or without feeding anemone’s vivo GDH activity began to rise in the first 12 dasy rising to 24 days volume has exceeded 100%, however, the GDH activity increased by 17% in the general state (light and feeding ), if host is in a completely dark environment, no matter there is no feeding or feeding, the activity will be high than the control ( p < 0.05 ), anemones average decrease of 59% total FAA and 26.4% EFAA in the darkness and feeding; in darkness without feeding will decrease on average 73.8% total FAA and76.8% EFAA. Another way we use of artificial to give carbon to replace lighting. The results are displayed in completely dark state feeding Artemia sp. or glycerol , comparing weight, protein count, alga density, photosynthetic capacity (Fv / Fm) and glutamate dehydrogenase of control (lighting and feeding) and between the two treatment after 24 days which all significant difference ( p < 0.05 ), compare two treatment (fed Artemia sp. and glycerol) and the control (lighting and feeding), two treatment were relatively low began to decrease from the fist 6 days to 24 days, its weight, protein count, alga density, photosynthetic capacity (Fv / Fm), respectively, reducing the weight of 30%, 42%; protein count of 52%, 65%; photosynthetic capacity (Fv / Fm) of 6%, 8% and alga density is reduced by 53%, 44%. Both treatment’s GDH activity is increased, which the activity of both increased by more than 100% ( p < 0.05 ), only malate dehydrogenase was no significant differences ( p > 0.05 ), from the above result, in the case of complete darkness caused by symbiotic algae can not photosynthesize, either not fed Artemia sp. or supply carbon, however the state of anemones compared with light status were have significant differences, so even to use of artificial means supplying additional nitrogen or carbon source, and as long as the symbiotic algae can not to carry out photosynthesis provide nutrition to the host, the host is still unable to maintain the light similar of physiological state.
| Identifer | oai:union.ndltd.org:TW/101TAJ00111006 |
| Date | January 2013 |
| Creators | Ya-Hui Liu, 劉雅惠 |
| Contributors | Jih-Terng Wang, 王志騰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
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