碩士 / 大仁科技大學 / 製藥科技研究所 / 101 / To evaluate the immunomodulatory effects of blue shark cartilage alkaline extract (BSC-AE) on ovalbumin (OVA)-sensitized and -challenged BALB/c mice. Experimental mice were divided into five groups, including non-sensitized control (code as PBS/DDW), dietary control (OVA/DDW), low dose BSC-AE supplement group (OVA/LD, 150 mg/kgBW/day), medium dose BSC-AE supplement group (OVA/MD, 450 mg/kgBW/day), and high dose BSA-AE supplement group (OVA/HD, 1350 mg/kgBW/day). The mouse allergic airway inflammation model was used in this study. Mice received 0.3 ml extra of deionized water or BSC-AE by tube gavage for five weeks. All experimental mice were sacrificed at day 35 and the was collected to determine the levels of pro-inflammatory (interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-6), anti-inflammatory (IL-10), T-helper type 1 (Th1) (IL-2 and interferon (IFN)-γ) and Th2 cytokines (IL-4 and IL-5) by ELISA assay. The results showed that BSC-AE supplement significantly (P < 0.05) decreased the cytokine levels but did not markedly (P > 0.05) affect the cytokine ratios of Th1/Th2 and pro-/anti-inflammatory cytokines. It is suggested that BSC-AE supplement may not modulate the Th1/Th2 balance toward Th1 pole in the allergic asthmatic murine model, but benefit to diminish the Th2-skewed allergic airway inflammation.
| Identifer | oai:union.ndltd.org:TW/101TAJ00549005 |
| Date | January 2013 |
| Creators | Mei-Chun Ting, 丁美純 |
| Contributors | Po-Chuen Shieh, Hui-Hsiang Chang, 謝博銓, 張惠香 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 48 |
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