碩士 / 大同大學 / 生物工程學系(所) / 101 / Davallia mariesii Moore ex Bak. is a medicinal, perennial fern. A rapid propagation protocol of Davallia mariesii has been established in this study. For callus induction, 0.1 g prothallus explants were cultured in 1/2 MS liquid medium supplemented with 2 % sucrose and subcultured in the same medium every month. Numbers of green callus was proliferated and the best growth ratio was 2.5 times after 2 months. Globular callus was formed from green callus in a diameter around 0.15 cm subcultured in the same medium every 2 weeks for 3 months. For sporophyte induction, globular callus in an average diameter of about 0.21 cm were cultured in 1/2 MS liquid medium supplemented with 2 % sucrose and 1 mg l-1 2ip (2-isopentenyl adenine). Sporophytes were regenerated and the percentage of induction rate was 100% after 2 months. The average number of regenerated sporophytes was about 35 per globular callus. The survival rate of sporophytes was about 70 % after acclimatization for 45 days. It is estimated that 1 g of prothallus explants as a starting inoculum can propagate more than 22,280 plants after one year. Regenerated sporophytes were diploid after chromosome examination.
| Identifer | oai:union.ndltd.org:TW/101TTU05106040 |
| Date | January 2013 |
| Creators | Kai-Ping Wang, 王凱平 |
| Contributors | Chin-Wen Ho, 何錦玟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 70 |
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