碩士 / 國立陽明大學 / 生物醫學影像暨放射科學系 / 101 / Objectives: Alzheimer’ disease (AD) is typified by deposition of β-amyloid (Aβ) within the brain accompany with cognitive decline. Imaging Aβ plaque could be useful for the development of the therapeutic and diagnosis target in AD. In this study, we aimed to access the change of Aβ and neuronal metabolism in the brain in J20 AD mice over age by using [11C]PIB and [18F]FDG PET.
Methods: The human amyloid precursor protein (hAPP) transgenic mouse lines J20 were bred and reared in our colony; littermates without hAPP were used as wild-type (wt) mice. The behavior test was applied to evaluate the change of memory and learning in APP mice. The animals (N=6/group) were imaged monthly from month 4 to month 12. The 20 min static imaging were performed after i.v. injection of [11C]PIB or [18F]FDG at 30 min. Thereafter, the brains of animals were removed for immunehistochemical staining for Aβ.
Results: The memory and learning performance was significantly deficit in APP mice after 5-6 months. The accumulation of [11C]PIB tended higher in APP mice than the that of wt mice since month 5. The age-dependent increased accumulation of [11C]PIB in APP mice appeared from month 5 to month 12. No significant difference of [18F]FDG uptake was observed between APP and wt mice in whole study period.
Conclusions: [11C]PIB PET could be used to distinguish APP mice from the control ones before onset at month 5 when pathological change of Aβ in hippocampus appears. Due to the limitation of micro PET imaging, the subtle change in the brain in earlier stage (<month 4) may not be detected. [18F]FDG PET does not show the significant metabolic deficits in APP mice when compared to wt mice. It could be implied that the neuron loss in APP mice happens later than the β-amyloid deposition.
| Identifer | oai:union.ndltd.org:TW/101YM005605028 |
| Date | January 2013 |
| Creators | Yu-Ning Tang, 唐于甯 |
| Contributors | Ren-Shyan Liu, 劉仁賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 65 |
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