碩士 / 國立中正大學 / 化學暨生物化學研究所 / 102 / Phthalates have been frequently used as plasticizers in personal care products and daily supplies. The exposure of phthalates may be through direct use or indirect means from environment. There are several types of phthalates, di(2-ethylhexyl) phthalate (DEHP) is the most widely found compound in vivo and Diet is believed to be the main source of DEHP in humans.In the first part of this thesis, the analysis and quantification of DEHP was by GC/FID. The biosorption of Dunaliella salina(D) and Chlorella pyrenoidosa (A, G) for DEHP were evaluated. Controlling variables are pH (at 2、7、8), algae dosage (mg/mL) and different DEHP concentrations (µg/mL). Results showed that equilibrium was reached after 20 hours when DEHP was with algae D, A, G. The biosorption was found to follow a pseudo second-order dynamic model and the biosorption behavior mmatches with Freundlich isotherm model. The maximum removal efficiency of DEHP was occurred at an optimum of pH 2.In the second part, further investigation on the effect of removal efficiency of DEHP by algae D, A, and G in the presence of bovine serum albumin (BSA) was conducted. Controlling variables are temperature 37 degree and 90 degree to simulate proteins in body temperature and proteins are thermally denatured. The interaction of DEHP to BSA or thermal-denatured BSA in aqueous solution has been measured by fluorescence spectra. The results revealed that DEHP caused quenching of BSA and thermal-denatured BSA fluorescence through a static quenching procedure. The binding constant (KA) between DEHP with BSA or thermal-denatured BSA had a great binding constant at pH 7 as (1.254±0.191)x10^5 and (0.546±0.127)x10^5 L/mol, respectively. Moreover, the analysis and quantitative adsorption efficiency of BSA and thermal-denatured BSA by algae D, A and G was determined by the enzyme-linked immunosorbent assay (ELISA).Results showed that BSA or thermal-denatured BSA equilibrium with algae was reached at 1 hour. Hence, DEHP could firstly form complexes with BSA or thermal-denatured BSA and then biosorption by algae D, A or G after 1 hour incubation to reach equilibrium. In the presence of BSA, the rate of DEHP removal was more rapid in comparison with algae (D, A, or G) alone.
In summary, this study exhibits the efficiency of Dunaliella and Chlorella pyrenoidosa as potential biosorbents for removal of DEHP. The biosorption behavior is different while bovine serum albumin (folded or thermally -denatured) was present. This phenomenon indicates that the algae (D, A, or G) not only have nutrient values in medical supplement but also contribute to biosorption of detrimental DEHP chemical once uptake.
| Identifer | oai:union.ndltd.org:TW/102CCU00065014 |
| Date | January 2014 |
| Creators | Chieh, Chan, 詹婕 |
| Contributors | Jong-Yuh Cherng, 程中玉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 108 |
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