碩士 / 長榮大學 / 職業安全與衛生學系碩士在職專班 / 102 / Recent researches have indicated that the antibiotic-resistant gene and pollutant-resistant gene are co-exist on plasmid and subjected to conjugative transfer from cell to cell. Under the selective pressure of anthropogenic pollutants, antibiotic-resistant pathogens might present in a higher population than in a related pristine environment. Considering the situations of dense human population and pig farms with unsatisfied wastewater treatment practice, research on how pollution affects the distribution of antibiotic resistant pathogens is deemed necessary. To explicit the relationship between the occurrence of resisting strains and the anthropogenic stress, this research conducted investigation on metal and antibiotic susceptibility of Vibrio spp. in the estuary of Erhjen River, characterized by its long history of metal pollution by the past scrap metal factories and recent industrial activities, in southern Taiwan. Vibrio spp. isolated from Chiku Lagoon in Tainan, a related pristine water body in Southern Taiwan, as well as strains from Beibin in Hwalian, seashore located at Eastern Taiwan, were compared to extend the spatial variability. The study assessed three metals (Cu, Zn, and Ni) and three antibiotics (gentamicin, ciprofloxacin, and tetracycline).
The study suggested, however, metal susceptibility of Vibrio spp. isolated from Erhjen River was unrelated to environmental stress with non-significant correlation coefficients (P>0.05) between minimum inhibitory concentrations (MIC) for Cu, Zn, Ni and sediment concentrations for the same metals. There was also no relationship found between metal resistance and antibiotic resistance for the Vibrio spp. isolated from the three sampling area. Correlation coefficients between MIC for heavy metals and MIC for antibiotics were non-significance (P>0.05). Comparing with Chiku and Beibin strains, Vibrio spp. isolated in Erhjen had significantly higher MICs for gentamicin and ciprofloxacin (Kruskal-Wallis ANOVA, P=0.008 and 0.007, respectively). All isolated Vibrio were resistance to tetracycline, suggesting ineffective curability of the antibiotic.
The preliminary study explored the ecology of Vibrio in a highly polluted environment. Attempts were made to understand the survival strategy of Vibrio in sediment with potential of heavy metal and antibiotic contaminations. The findings are expected not only benefit to the scientific communities but also to the policy makers who may use the information to protect the seafood consumption safety as well as to regulate the uses of antibiotics and heavy metals in the watershed.
| Identifer | oai:union.ndltd.org:TW/102CJU01590004 |
| Date | January 2014 |
| Creators | Shu-Chuan Chen, 陳淑娟 |
| Contributors | Horng, Ching-Yi, 洪慶宜 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 53 |
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