碩士 / 中山醫學大學 / 生化暨生物科技研究所 / 102 / Scratch gene play an important role in the regulation of neuron development. Recent study showed that downregulation of Scratch gene in mouse model impaired CNS differentiation. In present article, we want to examine whether knockdown of Scratch gene also cause the deficient in neurogenesis via zerbrafish model. According to the phylogenetic tree suggested zerbarfish scrt gene is high conserve with human and mouse counterpart. Based on PCR analysis and in situ hybridization assay we also founded that scrt gene is expressed from 36 hpf through 48 hpf, with relatively enrichment in adult zerbrafish brain. Knocking down of scrt gene by injection of anti-sense morpholino resulting in apoptosis in head at 24 hpf. In addition, there are massive apoptosis signal in eye region at 36 hpf. Moreover, disruption of scrt1b appeared more severe phenotype than scrt1a morphant. However, this effect could be restore via co-injection with scrt mRNA, implying the specificity of morpholino. Hence, these finding demonstrated a critical role of scrt gene not only in CNS but also in eye development.
| Identifer | oai:union.ndltd.org:TW/102CSMU5107017 |
| Date | January 2013 |
| Creators | KUAN-YING JIAN, 簡寬穎 |
| Contributors | 許立松 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 51 |
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