碩士 / 輔仁大學 / 生命科學系碩士班 / 103 / White spot syndrome virus (WSSV) is a serious pathogen of shrimp farming. For prevention, the farmer only depends on isolation and strengthens the immunity of shrimp. There is presently no remedy for shrimp’s white spot syndrome. Photodynamic treatment is a useful therapy for dental and tumor. It can conquer or kill widespread pathogens. The aim of this study is developing the aquacultural Photodynamic treating system to inhibit the activity of WSSV which was inside the shrimp. WSSV in this research was produced by Procambarus Clarkia which was infected by muscular-injection after 72h and extracted from muscle and gill. After titration, 1.5 fold LD50 of WSSV was injected into Penaeus vannamei with intramuscular injection, and sixty-two percentage of infected P. vannamei was dead in seven days. After PCR analyzed for WSSV’s VP22 gene fragment, all of the lethal shrimps had the positive signal. In order to inactive the WSSV in vivo. The infected shrimps were soaked with 1mM of methylene blue (MB) after infection and illuminated with 660nm, 525 mW / cm2 of LED for three minutes. The survival rate was increased to 17%. If the MB is injected into the infected shrimp and followed with same illumination conditions, the survival rate were up to 58% to the untreated group. These results show that the new developed aquacultural photodynamic treatment has potential to kill or inhibit WSSV in vivo.
| Identifer | oai:union.ndltd.org:TW/102FJU00105016 |
| Date | January 2015 |
| Creators | Cheng, Yu-Shun, 鄭渝勳 |
| Contributors | Li, Si-Shen, 李思賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 84 |
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