碩士 / 弘光科技大學 / 環境工程研究所 / 102 / The primary objectives of this study are to investigate the acute biotoxicity indicators using algae assay procedure bottle test method. The measuring algae and cyanobacteria including Chlorella, Microcystin, Anabeana, Oscillatoria, Gloeotrichia, and Nostoc. The test toxins were copper sulfate (CuSO4), cadmium chloride (CdCl2) and sodium dodecyl sulfate (SDS), which were prepared in raw water. The chlorophyll-a, cells number (cell density) and cell viability as toxicity parameters. Stress produced by the toxins addition was analyzed by flow cytometry. Experimental results show that the detection of a variety of algae chlorophyll a half inhibitory effective concentration (Effective Concentration 50%, referred to as EC50) of EC50 and algal density indicators show the differences between the sexual considerable, mainly because of the accumulation of the algal cells amount of poison may interfere with the test measurement of chlorophyll a, resulting in higher EC50 calculated error. In addition, the use of algae and algal cell counts stained with and the number of dead cells by flow cytometry in vivo measurements of the statistical results of the EC50, EC50 and staining of cell density count is closer.
After another for a variety of algae and toxic exposure 24hr, algae solution of soluble organic carbon (DOC) were significantly increased, presumably the result of this poison algae cells and the extracellular release of the reaction product of (Extracellular Polymeric Substances, EPS). For this experiment, CuSO4 and CdCl2 may also adsorbed or absorbed in the body algae speculated that the use of microwave digestion and extraction separation processing, Cu and Cd content analysis of the body of algae, found that addition of 35 to 90 % of the algal cells accumulate in the other a scanning electron microscope, and elemental analysis spectra (SEM-Mapping) analysis, also found in vivo with Cu metal alginate dispersed. Furthermore, the algal cells Comet (comet assay) was observed toxic algal cells are damaged, the extent of the various algae species have considerable differences. Thus found that the use of algae hydrostatic type analysis and detection of acute toxicity to aquatic organisms indicators to measure the number of algae (algae density) changes over the chlorophyll a is accurate, mainly because the latter would be subject to interference poisons.
| Identifer | oai:union.ndltd.org:TW/102HKU05515009 |
| Creators | WU, YI-UEN, 吳宜晏 |
| Contributors | 黃文鑑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 102 |
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