碩士 / 義守大學 / 生物科技學系 / 102 / In previous study, marine sponges have been found to be a rich source of potential bioactive natural products. To date, there are three therapeutic agents (two anticancer and one antiviral) that derive from the marine sponges. In order to search for bioactive metabolites, we have studied the chemical constituents from the EtOAc extract of a sponge Theonella sp. This study had led to the isolation of seven natural compounds 1–7, including four 4-methylene sterols (1–4), two nucleosides (5 and 6) and one macrolide (7). Except for the known compounds 2–4, 6 and 7, the acetyltheonellasterol F (1) was obtained as a new compound, while 5''-O-acetyl-2''-deoxyuridine (5) was found to be new natural product. The structures of these compounds were elucidated on the basis of their spectroscopic data.
The cytotoxicity of compounds 1–7 against the MCF-7 and MDA-MB-231 (human breast adenocarcinoma), T-47D (human hormone-dependent breast cancer), HCT-116 and DLD-1 (human colon adenocarcinoma), K562 (human chronic myelogenous leukemia) and Molt 4 (human T lymphoblast, acute lymphoblastic leukemia) tumor cell lines were determined. The results showed that compound 7, the most potent of compounds 1–7, exhibited cytotoxicity against MCF-7, T47D, HCT-116, DLD-1, K562 and Molt 4, with IC50s of 1.03, 0.65, 0.78, 0.55, 0.01 and 0.02 μg/mL, respectively. Furthermore, the antioxidant activity of compounds 1–7 was also tested. The results showed that compounds 1–7 have been shown to be not antioxidant activity.
| Identifer | oai:union.ndltd.org:TW/102ISU05111005 |
| Date | January 2014 |
| Creators | Yi-Long Sun, 孫藝龍 |
| Contributors | Ming-Cheng Shih, 石明正 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 148 |
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