碩士 / 高雄醫學大學 / 天然藥物研究所 / 102 / The clinical treatment of breast cancer has had great progress in recent years. Accumulating evidence has shown that cancer stem cells are crucial to cancer development. Therefore, the study on specific drugs against cancer stem cells is key to the future of cancer therapy. In this study, we have tested the effects of a marine algal extract on the suspension mammosphere cultures of breast cancer cell line MDA-MB-231.Treated MDA-MB-231 suspension cells showed decreased cell viability based on colorimetric WST1 assay. Flow cytometric analyses indicated that the algal extract treatment resulted in sub-G1 phase accumulation, increases in reactive oxygen species (ROS) , nitrous oxide species (NOS) levels and Annexin-V positive cells, as well as decreases in mitochondria membrane potential (MMP) in MDA-MB-231 suspension cells. These results indicated that this extract from a marine alga could decrease the survival of breast cancer stem cells via ROS induction and loss of mitochondria function. Soft agar assay and mammosphere assay showed that marine algal extract reduced MDA-MB-231 activity. Western blotting showed that anti-apoptosis proteins Bcl-2, Bcl-XL and breast cancer stem cell biomarker CD44 were decreased with treated extract. Anti-ER stress protein IRE-1α and CHOP were also decreased. Our finding may have clinical implication in suppressing breast cancer initiation and metastatis in the future.
| Identifer | oai:union.ndltd.org:TW/102KMC05068009 |
| Date | January 2014 |
| Creators | Ting-Wei Hung, 洪挺維 |
| Contributors | Chao-Neng Tseng, 曾昭能 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
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