碩士 / 國立高雄應用科技大學 / 化學工程與材料工程系博碩士班 / 102 / Biodegradable composites, based on PHA (poly(hydroxyalkanoate)) and MAP (marine algae powder) and their chemically-modified correspondences (PHA-g-AA and TMAP), were prepared and characterized to evaluate the possibility for biomedical application in this study. The acrylic acid (AA)-grafted PHA (PHA-g-AA) was obtained by chemically-grafting AA onto the PHA, while the coupling agent treated marine algae (TMAP) was obtained by mixing MAP with TEOS-based sol, which was acting as a compatiblizer, and dried. These composites were prepared by a blending process mixing the polymer (PHA or PHA-g-AA) and the reinforcement (MAP or TMAP) in a Brabender blender in a various ratios. NMR and IR were used to characterize the composite formation. After chemical characterization, the composites were subjected to several kinds of tests to evaluate their physical properties including mechanical property, surface morphology, water absorption, and biocompatibility. The experimental results showed: PHA-g-AA/TMAP composites had noticeably superior phase interface and envelopment, mechanical property and more homogeneous dispersion of TMAP in the PHA-g-AA matrix thereof when compared with those of PHA/MAP composites, because of possible ester linkage formation between the carboxyl group of PHA-g-AA polymer and the hydroxyl group of the reinforcement TMAP. The water resistance of PHA-g-AA/TMAP was higher than that of PHA/MAP about 2-6 wt%. To assess biocompatibility of these composites, normal human skin fibroblasts were seeded onto each of the composites. FB proliferation, collagen production, and cytotoxicity assays indicated that biocompatibility on the PHA/MAP composites were greater than on the PHA-g-AA/TMAP composites. Cell cycle and apoptosis revealed that DNAs of the cell were not seriously damaged, and deduced that cells were not affected by the composites and therefore appeared non-notable apoptosis and necrosis. Accordingly, these composites, PHA/MAP and PHA-g-AA/ TMAP, have a significant potential for the biomaterials application.
| Identifer | oai:union.ndltd.org:TW/102KUAS0063036 |
| Date | January 2014 |
| Creators | Chin-Yu Wang, 王晨羽 |
| Contributors | Fu-San Yen, 顏福杉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
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