碩士 / 銘傳大學 / 生物科技學系碩士班 / 102 / The Chlorella of lab number 1, 9, 11, 12, 13 ,and 14 were isolated from Ming Chuan University Taoyuan campus. Their ITS1 ( the internal transcribed spacer between 18s rDNA and 5.8s rDNA ) sequence alignment was used to study phylogenetic. No.1, 12, and 13 are same in the 273 base pairs, they are classified as the same species. Their ITS1 sequences have 99% similarity with Chlorella vulgaris, they are temporarily named as Chlorella vulgaris MCU01. No.9’s ITS has 273 bps, its sequence has 100% similarity with Chlorella sorokiniana, but has 19 bp different with no.1. No.11’s ITS1 has 273bps, its sequence has 20 and 2 bps different with no.1 and no.9, and has 99% similarity with Chlorella sorokiniana, it is temporarily named as Chlorella vulgaris MCU02. No.14’s ITS1 has 271 bps, its sequence has 97% similarity with Chlorella sorokiniana, it is temporarily named as Chlorella sorokiniana MCU03. No.14’s ITS sequence has 7 and 8 bps different with no.9 and no.11, and has 22 bps different with no1. No.7’s ITS has 243 bps, its sequence has 100% similarity with Parachlorella kessleri, to be classified as the same species. No.7 and former six kinds belong to different genus.
The solution was extracted by 80% methanol from fresh alga, which cultured to later stage on logarithmic phase of Chlorella vulgaris MCU01. The extraction use of concentrated under reduced pressure and then dissolved in ethanol solution, called to crude extract. Treatment was divided into three groups, they are crude extraction A, filtrated solution B ( crude extraction through the 0.22μm membrane filter), and supernatant C ( after centrifugation ). Then treat 48 hours on liver cell line AML12 and liver cancer cell line Huh7. When concentration on 0.1 g/L, except group B unaffected AML12 growth, the cell activity of group A and C were 88.4 and 87.3% in the control. In Huh7, the cell activity of group A was 89.5% in the control, the others unaffected Huh7 growth. On 0.2 g/L, three kind of treatments were inhibition AML12 growth and cell activity of group A、B and C were 70.1、88.4、73.5% respectively in the control. Except group B unaffected Huh7 growth, the cell activity of group A and C were 41.1 and 86.6% in the control. On 0.4 g/L, three kind of treatments will inhibit the growth of both cell. In AML12, the cell activity of group A、B and C were 48.6、83.3 and 48.2% in the control. In Huh7, the cell activity of group A、B and C were 25.3、58.3 and 54.4% in the control. On 0.8g/L , cellular activity of three treatment groups than in control were 35 and 15% or less in AML12 and Huh7. The LD50 of AML12 that treated 48 hours on crude extract A、filtrate B and supernatant C were 0.648, 1.145 and 0.753 g/L (dry/v) and Huh7 were 0.468, 0.727 and 0.712 g/L (dry/v).
| Identifer | oai:union.ndltd.org:TW/102MCU05111006 |
| Date | January 2014 |
| Creators | Jhin-Jie Chen, 陳志杰 |
| Contributors | Shu-Ling Chen, 陳淑玲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 86 |
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