碩士 / 明志科技大學 / 化學工程系生化工程碩士班 / 102 / The amount of phycobiliprotein is as high as 60% within Spirulina platens is cells. According to the pigment and absorption characteristics, the phycobiliprotein mainly includes C-phycocyanin (CPC) and allophycocyanin (APC). In this study, various functional groups onto the nanofiber membrane (e.g., dye-ligand, Histidine, and chitosan membranes) were obtained via different chemical synthesis. The adsorption characteristics for CPC and APC by membrane chromatography were assessed by measurements of the breakthrough curves.
Moreover, the membrane chromatography was used and evaluated the dynamic adsorption behavior for CPC in clarified S. platensis. All of the experiments were carried out with a membrane module and the dynamic adsorption behavior of the membranes was monitored using an AKTA Prime chromatographic system (GE Healthcare Biosciences). Firstly, the dye and histidine modified membrane were used to investigate the adsorption performance. Unfortunately, the results showed that the total protein (TP), CPC and APC broke through the membrane at the same time. This is due to the lack of selectively of these two types of membranes.
Hence, further experiments were carried out with chitosan modified membrane to examine the adsorption characteristics. The influences of NaCl concentration (0.1-1.0 M), adsorption pH (6-8), chitosan concentration (0.1-3.0 %), CPC concentration (0.05-0.50 mg/ml), and liquid flow rate (0.2-1.0 mL/min) on the adsorption performance of membrane for CPC (10 mL) were also investigated in membrane chromatography.
The results showed that the order of selectivity of chitosan-membrane for proteins was found to be TP > APC > CPC under the optimal operating conditions. In this case, APC and TP molecules were more easily adsorbed by the membrane. However, the CPC molecules would most easily penetrate the membrane without being adsorbed. Hence, the filtrate containing CPC would enhance its purity. The purification factor and total mass flux was found to be 3.3 folds and 66%, respectively. Under the circumstances, the optimal operating conditions were 0.1 M NaCl, 0.3 % disrupted cells, pH 7, and liquid flow rate 0.2 mL/min. Therefore, the chitosan modified membrane can be used to remove undesired proteins and further to enhance the purity of CPC.
| Identifer | oai:union.ndltd.org:TW/102MIT00723007 |
| Date | January 2014 |
| Creators | Zih-ming Ciou, 邱子銘 |
| Contributors | Yu-kaung Chang, 張煜光 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 77 |
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