碩士 / 國立中興大學 / 生命科學系所 / 102 / Taiwan is situated on the boundary separating the Eurasian plate to the west from the Philippine Sea Plate to the east. There were complex landforms from SW of Taiwan, such as ridges, mud volcanos, cold seeps and gas hydrate bearing regions. The isotopic data from Prof. Tsan-Yao Yang’s lab was suggested that most of the methane produced in Taiwan methane hydrate potential area is biogenic origin. To explore the methanogens at methane seeps or hydrate habitats, sediment samples obtained from piston core at Tainan Ridge, Good Weather Ridge, 96 Mud Volcano and Deformation Front by ORI (Ocean Reasearch I) cruises were enriched anaerobically. After anaerobic enrichment and serial sub-transfer, Methanoculleus sp. S3Fa from 96 Mud Volcano Groups was purified and characterized by MS Mei-Fei Chen. The 16S rRNA sequence of strain S3Fa showed 99% similarity with deep sea gas hydrate isolate Methanoculleus submarinus. Similar isolation methods were used in this study, three strains CYW1, CYW2 and CYW3, were purified from Deformation Front, Tainan Ridge and Good Weather Ridge, respectively. The 16S rRNA sequence of three strains showed 99% similarity with Methanosarcina mazei Go1. The whole-cell protein profile of strain CYW1, CYW2 and CYW3 are identical with M. mazei N2M9705. The irregular cocci cells of Methanoclleus sp. CYW4 was isolated from Defromation Front and showed 96-97% similarity with genus Methanoculleus. Strain CYW4 used H2 plus CO2 or formate as catabolic substrates. The optimum growth condition was 37°C, pH 8.02 and 0.08 M NaCl. The result showed that strain CYW4 was the novel new species and might be the new clade in genus Methanoculleus. It is expected that these methanogens isolated from methane seep and gas hydrate potential bearing regions contribute to the methane hydrate formation.
| Identifer | oai:union.ndltd.org:TW/102NCHU5105040 |
| Date | January 2014 |
| Creators | Chieh-Yin Weng, 翁杰愔 |
| Contributors | Mei-Chin Lai, 賴美津 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 91 |
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