碩士 / 國立成功大學 / 生物科技研究所 / 102 / The cytokine, TNF-α, is an important pro-inflammatory cytokine released by several immune cells during infection or tissue damage and is involved in a diverse range of inflammatory, infectious, and malignant conditions. TNF-α also has many functions in mammal, such as induces inflammation, macrophage activation, cell proliferation, apoptosis, and so on. In the previous studies, the functions of teleost TNF-α of fish were simular as mammal, such as increased expression of inflammatory genes. But, more than one TNF-α isoforms were found in teleost fish. Some of this isoforms was different in their amino acid or DNA sequences. In our privious study, we had found 2 orange-spotted grouper TNF genes: gTNF-α1 and gTNF-α2, they were only 38% amino acid identical. And they expressed different mRNA pattern in peripheral blood leukocytes (PBLs) under LPS stimulated, the mechanism is still unknow. In this study, we try to understand the different function in vivo. We analysis the gene expression of immunostimulant stimulated fish by rea-ltime PCR. The results show (1) tnf-α1 has shown a much stronger expression relative to tnf-α2 after LPS, Poly I:C, vibrio and nervous necrosis virus infected. (2) In various recombinated grouper cytokines stimulated fish, tnf-α1 has shown stronger expression than tnf-α2 after injection rgIL-1β and rgIFN-γ, but tnf-α2 seems to be a more significant factor than tnf-α1 after rgCCL4 injection. However, tnf-α1 and tnf-α2 both down expression after rg-IL-6. (3)TNF-α1 and TNF-α2 have shown the cross activation in each other. Overall, the results indicate that maybe tnf-α1 has more important role on innate immunity, and tnf-α2 is a helper to induce tnf-α1expression.
| Identifer | oai:union.ndltd.org:TW/102NCKU5111120 |
| Date | January 2014 |
| Creators | HsuanLin, 林炫 |
| Contributors | Han-You Lin, 林翰佑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 94 |
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