碩士 / 國立成功大學 / 生物科技研究所 / 102 / Until now, the mechanism of shrimp immune response still not fully understood. Previously, we found that shrimp has a hypervariable molecule called Down syndrome cell adhesion molecule (Dscam) which can recognize different pathogens. Based on Dscam structure and immune properties, Dscam has been proposed to be an antibody-like molecule in arthropod. In this study we use white spot syndrome virus (WSSV) as pathogen stimulator to observe the Dscam’s cytoplasmic tail variants expression in various tissue after WSSV infection. The results show that cytoplasmic tail element 1A which consist of an endocytosis motif was expressed mainly in immune-related tissues, while element 1B which lack of this motif and expressed mainly in non-immune related tissues.So, cytoplasmic tail element 1A may be involved in Dscam immune function. Furthermore, the membrane-bound and tail-less Dscam expressions were analyzed in various tissue after WSSV infection. In hemocyte, tail-less Dscam can be induced after 36 hour post WSSV infection. Thus, we hypothesized that high amount of tail-less Dscam can be secreted into hemolymph after pathogen challenge. In order to test the hypothesis, hemolymph from two months crayfish survivors after WSSV infection were collected. High level Dscam expression was detected in the WSSV survivor’s hemolymph. To investigate whether Dscam has similar property with IgM. We use gel filtration to analyze the possibility of Dscam forms polymer in hemolymph. The result shows that Dscam may form dimer or heterpolymer in the hemolymph. Taken together, based on Dscam cytoplasmic tail splicing mechanism, the secretion ability of Dscam after pathogen challenge and the polymer structure, this study suggested that Dscam may be an IgM-like moleculer in arthropod..
| Identifer | oai:union.ndltd.org:TW/102NCKU5111127 |
| Date | January 2014 |
| Creators | Jia-HungLin, 林家弘 |
| Contributors | Han-Ching Wang, 王涵青 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
Page generated in 0.013 seconds