碩士 / 國立成功大學 / 環境工程學系 / 102 / In this study, q-PCR and reverse transcription q-PCR methods were used to quantify total Microcystis gene (16S rRNA gene) and microcystin producing gene (mcyB gene), and the expression of the two targeted genes, respectively. To understand about the relationships among the Microcystis cell number, gene concentrations, gene expression, and microcystin concentrations, cell enumeration and microcystin were also analyzed using microscope and ELISA methods, respectively.The results of laboratory culture experiments indicated that the concentration of Microcystis cells has high correlation with those of the target genes. Gene copy number per cell was higher during the mid-log phase, and lower during the lag-phase. As grown time increased, microcystin produced on cell quata basis decreased, although no correlation was found between the producing gene copy number and the microcystin cell quata. For gene expression, both target genes expressed decreased as culturing time increased, with a few datum poitns increased during the log-phase. No correlation between gene expression and microcystin cocnentrations was found on cell quata basis. Nevertheless, a positive correlation was found between total gene expression and the net increase of total microcystin cocnentrations. The q-PCR methods were also applied in analysis of the environmental samples collected from Gourd Lake in Tainan. The results show that in the field samples the ratios of toxic Microcystis to total Microcystis were relatively low. However, the microcystin cell quota was quite high, mostly larger than 0.2 pg/cell as suggested by WHO.
| Identifer | oai:union.ndltd.org:TW/102NCKU5515039 |
| Date | January 2014 |
| Creators | Jia-ZeWeng, 翁嘉澤 |
| Contributors | Tsair-Fuh Lin, 林財富 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 95 |
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