碩士 / 國立彰化師範大學 / 生物學系 / 101 / This study investigated two kinds of Chlorella cultivated in lack of the different elements of N, P, Fe, Mg, Ca, and to measure the effect on its growth and lipid accumulation. When the algae were cultivated in the media seven days after exponential growth phase, then moved to the medium be removed a single element and incubated under identical environment conditions (temperature 25 ℃, light intensity 90-100 μmol/m2s, and 12:12 h light: dark) for 7 days. Continuous measurement of absorbance (optical density), the dry weight of biomass and direct counting method measured the growth of those two Chlorella, in addition using chlorophyll fluorescence PAM to measure the maximum quantum efficiency (Fv/Fm) to observe algae health conditions. Then take out 0.5 g dry weight of algae at the first, third and seventh days, and use the method of lipid drying weight to extracting lipid. Those two Chlorella cultivated in normal medium for 0-7 days, it has the maximum cell density, 0.35 and 0.39 g/L on day 7, respectively. The growth rates of those two Chlorella are decreasing as days increasing under the medium lack of single element, and the maximum quantum efficiency (Fv/Fm) showed significantly decreasing. The two kinds of Chlorella cultivated in the stress condition have decreased in the growth rate, and increased in the lipid contents. Chlorella sp. 1 has lipid accumulation on day 3, it has the highest lipid content about 3.4 % when cultivated under deprived of iron at day 5. Chlorella sp. 2 begin to accumulate lipid on day 5, and has the highest lipid content, 15.3 %, in deprived of iron on day 7. Although Chlorella sp. 2 obtain the best lipid content until day 7, but the totally lipid contents are more than Chlorella sp. 1. Therefore, Chlorella sp. 2 has the potential for extracting lipids as the oleaginous algae.
| Identifer | oai:union.ndltd.org:TW/102NCUE5112001 |
| Date | January 2013 |
| Creators | Fang-Yi Lin, 林芳伊 |
| Contributors | Wei-Lung Wang, 王瑋龍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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