碩士 / 國防醫學院 / 藥學研究所 / 102 / Over the past few decades, the emergence of multidrug-resistant bacteria continuously threatening public health has created an urgent need for alternative therapeutics. While the development of antimicrobial peptides (AMPs) have been considered as a promising strategy, protamine possessing prominent efficacy against a range of Gram-positive and Gram-negative bacteria shall be a potential target. A review of literatures indicates that low molecular weight protamines (LMWP) have significantly reduced toxicity, antigenicity and immunogenicity. Therefore, the aim of this study is to investigate the potential of LMWP as a new class of antibacterial peptides.
In this study, two types of LMWPs derived from salmine and clupeine via enzymatic digestion, denoted thermolysin-digested salmine protamines (TDSP) and thermolysin-digested clupeine protamines (TDCP), were prepared. They were characterized as arginine-rich sequences with molecular weights ranging from 0.7-1.8 kDa. While the major portion of TDSP was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to compose of TDSP3 (PRRRRSSSRRP) and TDSP5 (VSRRRRRRGGRRRR) peptides, TDSP5 showed high potency against Gram-negative strains including Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii. On the other hand, all protamine peptides exhibited very little hemolytic toxicity to human red blood cells.
Taken together, the chain-shortened protamine peptide such as TDSP5 showed the potential to develop as a new class of antibacterial peptide with broad-spectrum activity and low cytotoxicity against Gram-positive and Gram-negative bacteria.
| Identifer | oai:union.ndltd.org:TW/102NDMC0551008 |
| Date | January 2014 |
| Creators | Wang, Chih-An, 王智安 |
| Contributors | Chang, Li-Chien, 張立乾 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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