碩士 / 國立新竹教育大學 / 應用科學系碩士班 / 102 / Backgrounds:
Breast cancer is a life-threatening disease among women worldwide with annual rates of reported incidence and death increasing alarmingly. Standard cancer therapy generally combines surgery and multi therapeutic agents . However, These anticancer agents have severe side effects in drugs. Therefore, alternative interventions are required to replace or to supplement current regimens. An increasing number of literature evidence indicates that complementary and alternative medicine (CAM) used in treatment-related symptom control and alleviation of side effects plays an important role in increasing survival rate and quality of life in breast cancer patients. The leaf and seed of Aicennia marina Forssk. (A. marina) are used in traditional medicine to treat skin diseases. However, its anticancer activities have not been systematically studied. The main goal of this study is to investigate the effect extract of Avicennia marina on anti-cancer and pharmacologically active compounds.
Materials and Methods:
The A. marina extracts were extracted with ethanol, Ethyl acetate or water. The cell viability was evaluated by MTT assay. The effect of A. marina extracts on the induction of apoptosis in breast cancer cells by DNA fragmentation assay and flow cytometry. After treatment with A. marina extracts at the indicated concentrations, cells were harvested and protein levels of caspase-8, caspase-3, and PARP were determined by Western blotting. Furthermore, several fractions of ethyl acetate extracts purified by column chromatography, and we want to find out novel potential anti-cancer compounds.
Results:
The antitumor activity of A. marina extracts were evaluated in NIH 3T3, AU565, BT483, and MDA-MB-231 cell lines. We found that ethyl acetate extracts of A. marina have higher anticancer activity on human breast cancer cells compared with other extracts. Further studies revealed that ethyl acetate extract from A. marina induced cell apoptosis by caspase -3 activation. Phytochemical analysis showed that the extract is rich in phenolic compounds that are capable in inhibition of cancer cell growth by A. marina extracts.
Conclusion:
The experimental results suggest that the important cytotoxic compoents could be isolated from ethyl acetate extract of A. marina. Further studies are required to investigate A. marina potential as an anticancer therapeutic agent.
| Identifer | oai:union.ndltd.org:TW/102NHCT5115006 |
| Date | January 2013 |
| Creators | Jia Hua Chang, 張佳華 |
| Contributors | Dr. Hsiu Chen Huang, Dr. Cheng Huang, 黃琇珍, 黃琤 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 119 |
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