碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 102 / α-phellandrene (α-PA) is a kind of cyclic monoterpenes compounds, which is a common active principle from essential oil of dietary specie or herb. α-PA active has been shown antibacterial, antioxidant, anticancer, hypolipidemic and improve immune of function. To investigate the regulatory roles of α-PA in physiological and immune responses in shrimp, we examined innate physiological responses (including haemolymph glucose, lactate, triglycerides and protein), non-specific immune responses (including phenoloxidase (PO), respiratory bursts (RB), superoxide dismutase (SOD) activity and total haemocyte count (THC)) in white shrimp (Litopenaeus vannamei) that were individually injected with the α-PA at 4, 8, and 12 µg g-1. The results indicated shrimp injected with the α-PA can maintain stability glucose, lactate, and protein levels. PO activity of shrimps receiving the α-PA at 12 µg g-1 significantly increased at 12 hour. Shrimp receiving the α-PA had significantly increased RB activity at 12 hour, particularly 8 µg g-1 had significantly increased. The SOD activity and THC of shrimp that received the α-PA at 12 μg g-1 was significantly higher at 12 hour. The survival rate of shrimp injected with the α-PA at 8 µg g-1 was higher than that of shrimp injected saline, their enhanced resistance against white spot syndrome virus (WSSV) infections.
This study suggests that injected with the α-PA of 8 and 12 µg g-1 at 12 hour can enhance immunity and not change physiological homeostasis in white shrimp.
| Identifer | oai:union.ndltd.org:TW/102NKIM0084007 |
| Date | January 2014 |
| Creators | Chia-Ling Lin, 林嘉凌 |
| Contributors | Shu-Ling Hsieh, 謝淑玲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 116 |
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